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Entry from: NCCB_PHAGES
 
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CABRI:NCCB_PHAGES
Collection_number NCCB 3037
Name Charon35
Type phage
Other_collection_numbers PC-V3037
Restricted_distribution No
Literature Loenen, W.A.M. et al., Gene (1983) 26:171-179 [PMID: 6323258 ]
History_of_deposit -
Host_for_propagation Escherichia coli LamB+, lambda-
Host_used_for_propagation Escherichia coli DH1 = NCCB 2956
Lysogenicity No
Virus_used_for -
Cell_surface_receptor LamB
Medium Medium 171
Class Recombinant
Plaque_forming_phage No
Clear_plaque_morphology No
Minimum_packaging_capacity 9 kb
Maximum_packaging_capacity 18.6 kb
Nucleic_acid_type DNA,double stranded,linear
Further_information properties of recombinants: cI-, Gam+, Int-, Red-; two extra BamHI sites are present in the stuffer fragment, enabling the stuffer fragment to be readily separated from the phage arms after digestion of the vector DNA with BamHI lambda replacement vector
Restriction_sites ():BamHI,EcoRI,HindIII,SacI,SalI,XbaI
Price_code 1
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